验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human ovary tumor tissue, rat eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
15773-1-AP targets VAX2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | VAX2 fusion protein Ag8439 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ventral anterior homeobox 2 |
| Synonyms | DRES93, VAX2, ventral anterior homeobox 2 |
| Calculated Molecular Weight | 290 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC006336 |
| Gene Symbol | VAX2 |
| Gene ID (NCBI) | 25806 |
| RRID | AB_2878183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIW0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ventral anterior homeobox 2 (VAX2) has been reported to be specifically expressed in the ventral region of the prospective neural retina in vertebrates and plays crucial roles in regulating the proper development of the ventral region of eye (PMID: 21148184). VAX2 inactivatiin mouse leads to alterations in the establishment of a proper dorsoventral eye axis during embryonic development (PMID: 33817157).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAX2 antibody 15773-1-AP | Download protocol |
| IHC protocol for VAX2 antibody 15773-1-AP | Download protocol |
| IP protocol for VAX2 antibody 15773-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |