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VBP1 Polyclonal antibody

VBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14345-1-AP

Print datasheet

Synonyms

von Hippel-Lindau-binding protein 1, VHL-binding protein 1, VBP-1, PFDN3, PFD3

验证数据展示

  • WB analysis using 14345-1-AP

    WB analysis using 14345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:8000 incubated at 4 degree celsius over night.

  • IHC staining of human thyroid cancer using 14345-1-AP

    IHC staining of human thyroid cancer using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VBP1 antibody included
  • IHC staining of human thyroid cancer using 14345-1-AP

    IHC staining of human thyroid cancer using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VBP1 antibody included
  • IF Staining of HepG2 using 14345-1-AP

    IF Staining of HepG2 using 14345-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VBP1 antibody (14345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inC6 cells, K-562 cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VBP1 Ready-To-Use IHC Kit. VBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14345-1-AP targets VBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Immunogen VBP1 fusion protein Ag5685 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name von Hippel-Lindau binding protein 1
Synonyms von Hippel-Lindau-binding protein 1, VHL-binding protein 1, VBP-1, PFDN3, PFD3
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC046094
Gene Symbol VBP1
Gene ID (NCBI) 7411
RRIDAB_2213910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61758
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VBP1 antibody 14345-1-APDownload protocol
IHC protocol for VBP1 antibody 14345-1-APDownload protocol
IF protocol for VBP1 antibody 14345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

PLoS Genet

The prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation.

Authors - Franck Chesnel
View Article