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验证数据展示

  • WB analysis using 14345-1-AP

    WB analysis using 14345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:8000 incubated at 4 degree celsius over night.

  • IHC staining of human thyroid cancer using 14345-1-AP

    IHC staining of human thyroid cancer using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VBP1 antibody included
  • IHC staining of human thyroid cancer using 14345-1-AP

    IHC staining of human thyroid cancer using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VBP1 antibody included
  • IF Staining of HepG2 using 14345-1-AP

    IF Staining of HepG2 using 14345-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VBP1 antibody (14345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

VBP1 Polyclonal antibody

VBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14345-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

von Hippel-Lindau-binding protein 1, VHL-binding protein 1, VBP-1, PFDN3, PFD3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, K-562 cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VBP1 Ready-To-Use IHC Kit. VBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14345-1-AP targets VBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
免疫原 VBP1 fusion protein Ag5685 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 von Hippel-Lindau binding protein 1
别名 von Hippel-Lindau-binding protein 1, VHL-binding protein 1, VBP-1, PFDN3, PFD3
计算分子量 23 kDa
观测分子量 23-25 kDa
GenBank蛋白编号BC046094
基因名称 VBP1
Gene ID (NCBI) 7411
RRIDAB_2213910
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61758
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VBP1 antibody 14345-1-APDownload protocol
IHC protocol for VBP1 antibody 14345-1-APDownload protocol
IF protocol for VBP1 antibody 14345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

PLoS Genet

The prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation.

Authors - Franck Chesnel
View Article