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验证数据展示

  • WB analysis using 30958-1-AP

    WB analysis using 30958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30958-1-AP (VCAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 30958-1-AP

    IHC staining of mouse spleen using 30958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 30958-1-AP

    IHC staining of mouse spleen using 30958-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30958-1-AP (VCAM-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 30958-1-AP

    IF Staining of mouse spleen using 30958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 30958-1-AP

    IF Staining of mouse spleen using 30958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using VCAM-1 antibody (30958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VCAM-1/CD106 Polyclonal antibody

VCAM-1/CD106 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 30958-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

CD106, VCAM-1, Vcam1, Vcam 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat spleen tissue, mouse spleen tissue, C2C12 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30958-1-AP targets VCAM-1/CD106 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 mouse, rat
文献引用反应性mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 CD106, VCAM-1, Vcam1, Vcam 1
计算分子量81 kDa
观测分子量100-110 kDa
GenBank蛋白编号CAA47989.1
基因名称 VCAM-1
Gene ID (NCBI) 22329
RRIDAB_3086429
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
WB protocol for VCAM-1/CD106 antibody 30958-1-APDownload protocol
IHC protocol for VCAM-1/CD106 antibody 30958-1-APDownload protocol
IF protocol for VCAM-1/CD106 antibody 30958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Microvasc Res

Characterization of microcirculatory endothelial functions in a D-Galactose-induced aging model

Authors - Zhuo Li
View Article
mouseIF

Redox Biol

Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation

Authors - Hui He
View Article