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验证数据展示

  • WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    IHC staining of human tonsillitis using 84249-4-RR (same clone as 84249-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCAM-1 antibody included
  • Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3, Human VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 84249-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02345. 84249-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01145-3

    Sample test of MP01145-3

    Serum of six individual healthy human donors was measured. The human VCAM-1/CD106 concentration of detected samples was determined to be 188.79 ng/mL with a range of 126.68 - 336.06 ng/mL.

  • Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3, VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84249-4-PBS. Detection antibody: 84249-2-PBS. Standard: RP02345. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84249-4-RR

    Affinity and Kinetic Characterization of 84249-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84249-4-RR against Human VCAM-1/CD106 were performed. The affinity constant is 2.18 nM.

VCAM-1/CD106 Recombinant antibody, PBS Only (Capture)

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84249-4-PBS

产品说明书

CloneNo. 241284B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

产品信息

84249-4-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP01145-3: 84249-4-PBS capture and 84249-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular cell adhesion molecule 1
别名 VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100
计算分子量81 kDa
观测分子量100 kDa
GenBank蛋白编号NM_001078.4
基因名称 VCAM-1
Gene ID (NCBI) 7412
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19320-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).