• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30599-1-AP

    WB analysis using 30599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30599-1-AP (VCAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 30599-1-AP

    IHC staining of human endometrial cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 30599-1-AP

    IHC staining of human endometrial cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30599-1-AP

    IHC staining of human stomach cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30599-1-AP

    IHC staining of human stomach cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 30599-1-AP

    IF Staining of NIH/3T3 using 30599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 30599-1-AP

    IF Staining of NIH/3T3 using 30599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VCAN Polyclonal antibody

VCAN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30599-1-AP targets VCAN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 VCAN fusion protein Ag30151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 versican
别名 PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR
计算分子量 373 kDa
观测分子量250-300 kDa, 550 kDa
GenBank蛋白编号NM_004385
基因名称 VCAN
Gene ID (NCBI) 1462
RRIDAB_3669736
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13611
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).

实验方案

Product Specific Protocols
WB protocol for VCAN antibody 30599-1-APDownload protocol
IHC protocol for VCAN antibody 30599-1-APDownload protocol
IF protocol for VCAN antibody 30599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols