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VCPIP1 Polyclonal antibody

VCPIP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17802-1-AP

Print datasheet

Synonyms

DUBA3, KIAA1850, VCIP135, VCPIP1

验证数据展示

  • IHC staining of human ovary tumor using 17802-1-AP

    IHC staining of human ovary tumor using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17802-1-AP (VCPIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 17802-1-AP

    WB analysis of MCF-7 using 17802-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17802-1-AP (VCPIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17802-1-AP

    IHC staining of human breast cancer using 17802-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17802-1-AP (VCPIP1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMCF7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17802-1-AP targets VCPIP1 in WB, IHC, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen VCPIP1 fusion protein Ag12035 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name valosin containing protein (p97)/p47 complex interacting protein 1
Synonyms DUBA3, KIAA1850, VCIP135, VCPIP1
Calculated Molecular Weight 1222 aa, 134 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC094799
Gene Symbol VCPIP1
Gene ID (NCBI) 80124
RRIDAB_10699873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JH7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VCPIP1 antibody 17802-1-APDownload protocol
IHC protocol for VCPIP1 antibody 17802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Immunol Res

USP22 deubiquitinates CD274 to suppress anti-cancer immunity.

Authors - Xing Huang
View Article
humanRIP

Genes Dis

Downregulation of nc886 contributes to prostate cancer cell invasion and TGFβ1-induced EMT.

Authors - Ronghui Yang
View Article