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验证数据展示

  • WB analysis of HepG2 using 55261-1-AP

    WB analysis of HepG2 using 55261-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55261-1-AP

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with VDAC2 antibody included
  • IHC staining of human kidney using 55261-1-AP

    IHC staining of human kidney using 55261-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with VDAC2 antibody included

VDAC2 Polyclonal antibody

VDAC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55261-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hVDAC2, VDAC 2, VDAC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, HuH-7 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55261-1-AP targets VDAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 voltage-dependent anion channel 2
别名 hVDAC2, VDAC 2, VDAC2
计算分子量 33 kDa
观测分子量 31-33 kDa
GenBank蛋白编号NM_003375
基因名称 VDAC2
Gene ID (NCBI) 7417
RRIDAB_10852961
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45880
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

实验方案

Product Specific Protocols
WB protocol for VDAC2 antibody 55261-1-APDownload protocol
IHC protocol for VDAC2 antibody 55261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Gasdermin D-mediated metabolic crosstalk promotes tissue repair

Authors - Zhexu Chi
View Article
humanWB

Cell Physiol Biochem

Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.

Authors - Meiying Yang
View Article