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VDAC2 Recombinant antibody, PBS Only

VDAC2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241569C3

Cat no : 84225-3-PBS

Print datasheet

Synonyms

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3

验证数据展示

  • IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VDAC2 antibody (84225-3-RR, Clone: 241569C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VDAC2 antibody included
  • FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-3-RR, Clone:241569C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

产品信息

The immunogen of 84225-3-PBS is VDAC2 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen VDAC2 fusion protein Ag2266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name voltage-dependent anion channel 2
Synonyms VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3
Calculated Molecular Weight 294 aa, 32 kDa
GenBank Accession NumberBC000165
Gene Symbol VDAC2
Gene ID (NCBI) 7417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP45880
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)