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验证数据展示

  • IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    IF Staining of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VDAC2 antibody (84225-3-RR, Clone: 241569C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    IHC staining of mouse heart using 84225-3-RR (same clone as 84225-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84225-3-RR (VDAC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VDAC2 antibody included
  • FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    FC experiment of HeLa using 84225-3-RR (same clone as 84225-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vdac2 Recombinant Antibody (84225-3-RR, Clone:241569C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84225-3-PBS in a different storage buffer formulation.

VDAC2 Recombinant antibody, PBS Only

VDAC2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84225-3-PBS

产品说明书

CloneNo. 241569C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VDAC2 Ready-To-Use IHC Kit. VDAC2 primary antibody included.

产品信息

84225-3-PBS targets VDAC2 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 VDAC2 fusion protein Ag2266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 voltage-dependent anion channel 2
别名 VDAC-2, VDAC 2, hVDAC2, FLJ23841, 241569C3
计算分子量 294 aa, 32 kDa
GenBank蛋白编号BC000165
基因名称 VDAC2
Gene ID (NCBI) 7417
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP45880
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and especially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue. (PMID: 22020053)