验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue, human blood, rat eye tissue, mouse eye tissue, PC-3 cells, human plasma, mouse testis tissue |
| Positive IHC detected in | human liver tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16922-1-AP targets Vitamin D binding protein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 | Vitamin D binding protein fusion protein Ag10286 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | group-specific component (vitamin D binding protein) |
| 别名 | GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf |
| 计算分子量 | 474 aa, 53 kDa |
| 观测分子量 | 52-58 kDa |
| GenBank蛋白编号 | BC057228 |
| 基因名称 | Vitamin D binding protein |
| Gene ID (NCBI) | 2638 |
| RRID | AB_10597098 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02774 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Vitamin D binding protein antibody 16922-1-AP | Download protocol |
| IHC protocol for Vitamin D binding protein antibody 16922-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Methods Clin Dev Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice | ||
Proteomics Screening for potential serum biomarkers in rat mesangial proliferative nephritis. | ||
Pharmaceutics Evaluation of In Vitro and In Vivo Antiviral Activities of Vitamin D for SARS-CoV-2 and Variants | ||
Int Immunopharmacol Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function |
