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验证数据展示

  • WB analysis of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    WB analysis of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66804-1-Ig (VE-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    IF Staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 66804-1-Ig (same clone as 66804-1-PBS)

    IF Staining of HUVEC using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunofluorescent analysis of un-fixed HUVEC cells using VE-cadherin/CD144 antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

VE-cadherin/CD144 Monoclonal antibody, PBS Only

VE-cadherin/CD144 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66804-1-PBS

产品说明书

CloneNo. 4F9B10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66804-1-PBS targets VE-cadherin/CD144 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 VE-cadherin/CD144 fusion protein Ag27501 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 5, type 2 (vascular endothelium)
别名 VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen
计算分子量 88 kDa
观测分子量125 kDa, 100 kDa
GenBank蛋白编号NM_001795
基因名称 VE-cadherin
Gene ID (NCBI) 1003
RRIDAB_2882147
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP33151
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).