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验证数据展示

  • WB analysis using 31772-1-AP

    WB analysis using 31772-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31772-1-AP (VE-cadherin/CD144 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 31772-1-AP

    IF Staining of HUVEC using 31772-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (31772-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VE-cadherin/CD144 Polyclonal antibody

VE-cadherin/CD144 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31772-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

VE-cadherin, Vascular endothelial cadherin, CDH5, CD144, Cadherin 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, human placenta tissue
Positive IF/ICC detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31772-1-AP targets VE-cadherin/CD144 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin 5, type 2 (vascular endothelium)
别名 VE-cadherin, Vascular endothelial cadherin, CDH5, CD144, Cadherin 5
计算分子量88 kDa
观测分子量90-100 kDa, 130-140 kDa
GenBank蛋白编号NM_001795.5
基因名称 VE-cadherin
Gene ID (NCBI) 1003
RRIDAB_3670108
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP33151-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

实验方案

Product Specific Protocols
WB protocol for VE-cadherin/CD144 antibody 31772-1-APDownload protocol
IF protocol for VE-cadherin/CD144 antibody 31772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols