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验证数据展示

  • WB analysis using 81323-2-RR

    WB analysis using 81323-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81323-2-RR (VEGFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81323-2-RR

    IF Staining of HeLa using 81323-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (81323-2-RR, Clone: 7H10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 81323-2-RR

    Affinity and Kinetic Characterization of 81323-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81323-2-RR against Human VEGFA were performed. The affinity constant is 1.60 nM.

VEGFA Recombinant antibody

VEGFA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81323-2-RR

产品说明书

CloneNo. 7H10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, A431 cells, MDA-MB-231 cells, HUVEC cells, RAW 264.7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81323-2-RR targets VEGFA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 VEGFA fusion protein Ag13500 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF
计算分子量 412 aa, 46 kDa
观测分子量40 kDa
GenBank蛋白编号BC065522
基因名称 VEGFA
Gene ID (NCBI) 7422
RRIDAB_3086457
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 81323-2-RRDownload protocol
IF protocol for VEGFA antibody 81323-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols