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VEGFA Recombinant antibody

VEGFA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7H10

Cat no : 81323-2-RR

Print datasheet

Synonyms

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

验证数据展示

  • WB analysis using 81323-2-RR

    WB analysis using 81323-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81323-2-RR (VEGFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81323-2-RR

    IF Staining of HeLa using 81323-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (81323-2-RR, Clone: 7H10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 81323-2-RR

    Affinity and Kinetic Characterization of 81323-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81323-2-RR against Human VEGFA were performed. The affinity constant is 1.60 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, A431 cells, MDA-MB-231 cells, HUVEC cells, RAW 264.7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81323-2-RR targets VEGFA in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen VEGFA fusion protein Ag13500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF
Calculated Molecular Weight 412 aa, 46 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC065522
Gene Symbol VEGFA
Gene ID (NCBI) 7422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 81323-2-RRDownload protocol
IF protocol for VEGFA antibody 81323-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols