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验证数据展示

  • IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

SLC32A1/VGAT Polyclonal antibody, PBS Only

SLC32A1/VGAT Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 14471-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

SLC32A1, VGAT, VIAAT, Vesicular GABA transporter, hVIAAT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14471-1-PBS targets SLC32A1/VGAT in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SLC32A1/VGAT fusion protein Ag5843 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 32 (GABA vesicular transporter), member 1
别名 SLC32A1, VGAT, VIAAT, Vesicular GABA transporter, hVIAAT
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC053582
基因名称 VGAT
Gene ID (NCBI) 140679
RRIDAB_10644324
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H598
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.