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VGAT Polyclonal antibody, PBS Only

VGAT Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14471-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IHC staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    WB analysis of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 14471-1-AP (VGAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP experiment of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IP result of anti-VGAT (IP:14471-1-AP, 4ug; Detection:14471-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    IHC staining of mouse brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14471-1-AP (VGAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    IF Staining of rat brain using 14471-1-AP (same clone as 14471-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using VGAT antibody (14471-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14471-1-PBS in a different storage buffer formulation.

产品信息

14471-1-PBS targets VGAT in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen VGAT fusion protein Ag5843 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC053582
Gene Symbol VGAT
Gene ID (NCBI) 140679
RRIDAB_10644324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H598
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC32A1, also known as VGAT (vesicular GABA transporter), functions in the uptake of GABA and glycine into synaptic vesicles. GABA (gamma-aminobutyric acid), is the major inhibitory neurotransmitter in the CNS. VGAT transports GABA and glycine into acidic vesicles and localizes to the synaptic vesicle in glycinergic and GABAergic neurons. And VGAT antibodies are useful markers for presynaptic GABAergic and glycinergic neurons.