• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VGLUT2 Polyclonal antibody

VGLUT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29209-1-AP

Print datasheet

Synonyms

SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI

验证数据展示

  • WB analysis using 29209-1-AP

    WB analysis using 29209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29209-1-AP (VGLUT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29209-1-AP

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29209-1-AP

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 29209-1-AP

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 29209-1-AP

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29209-1-AP targets VGLUT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen VGLUT2 fusion protein Ag29565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberNM_020346
Gene Symbol VGLUT2
Gene ID (NCBI) 57084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

实验方案

Product Specific Protocols
WB protocol for VGLUT2 antibody 29209-1-APDownload protocol
IHC protocol for VGLUT2 antibody 29209-1-APDownload protocol
IF protocol for VGLUT2 antibody 29209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols