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Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for FC, IF/ICC,IF-Fro,IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)


FC, IF/ICC,IF-Fro,IF-P, IHC, WB, ELISA and More (3)



Cat No : 10366-1-AP

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VIM, vimentin


Positive WB detected inHeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human liver tissue, human hepatocirrhosis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human breast cancer tissue, human kidney tissue
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.


Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


10366-1-AP targets Vimentin in WB, IP, IF, FC, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC,IF-Fro,IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, FC, IHC, CoIP, ELISA, Cell treatment
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, hamster, pig, bovine
Immunogen Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vimentin
Synonyms VIM, vimentin
Calculated Molecular Weight 466 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC000163
Gene Symbol VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).


Product Specific Protocols
WB protocol for Vimentin antibody 10366-1-APDownload protocol
IHC protocol for Vimentin antibody 10366-1-APDownload protocol
IF protocol for Vimentin antibody 10366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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