验证数据展示

WB analysis using 10366-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Vimentin antibody included
WB analysis of Jurkat cells using 10366-1-AP
WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.
IF Staining of human breast cancer using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human kidney using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse brain using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10366-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 10366-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Vimentin (10366-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of human breast cancer using 10366-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human colon using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10366-1-AP (Vimentin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Vimentin antibody included
IHC staining of human hepatocirrhosis using 10366-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Vimentin antibody included
IF Staining of HepG2 using 10366-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (10366-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of mMSCs using 10366-1-AP
1x10^6 mMSCs cells were intracellularly stained with 0.5 ug Vimentin Polyclonal antibody (10366-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 10366-1-AP

Publications(1749)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA and More (5)

Synonyms

VIM

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (1749)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	HepG2 cells, NIH/3T3 cells, mouse heart tissue, Jurkat cells, HeLa cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue, human kidney tissue
Positive IF-Fro detected in	mouse brain tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells, mMSCs cells

Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 1458 publications below
IHC	See 278 publications below
IF	See 351 publications below
IP	See 9 publications below
ELISA	See 1 publications below
FC	See 2 publications below
CoIP	See 5 publications below
RIP	See 1 publications below

产品信息

10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
Cited Applications	WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine, hamster, sheep, goat, deer
Immunogen	Vimentin fusion protein Ag0489 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	vimentin
Synonyms	VIM
Calculated Molecular Weight	466 aa, 54 kDa
Observed Molecular Weight	54 kDa
GenBank Accession Number	BC000163
Gene Symbol	VIM
Gene ID (NCBI)	7431
ENSEMBL Gene ID	ENSG00000026025
RRID	AB_2273020
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08670
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

实验方案

Product Specific Protocols
WB protocol for Vimentin antibody 10366-1-AP	Download protocol
IHC protocol for Vimentin antibody 10366-1-AP	Download protocol
IF protocol for Vimentin antibody 10366-1-AP	Download protocol
FC protocol for Vimentin antibody 10366-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Nat Cancer Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma Authors - Xinyao Qiu View Article
human	WB	Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Authors - Jia-Yuan Zhang View Article
human	WB	ACS Nano Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition Authors - Wenjie Wu View Article
mouse	IF	Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation Authors - Zhenyu Tian View Article
human		Adv Sci (Weinh) PMID: 31728273 Authors - Qingming Tang View Article
	IHC	Circ Res Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis. Authors - Jiangbin Wu View Article

验证数据展示

WB analysis using 10366-1-AP

IHC staining of human liver cancer using 10366-1-AP

WB analysis of Jurkat cells using 10366-1-AP

IF Staining of human breast cancer using 10366-1-AP

IF Staining of human kidney using 10366-1-AP

IF Staining of mouse brain using 10366-1-AP

IF Staining of HepG2 using 10366-1-AP

FC experiment of HeLa using 10366-1-AP

IF Staining of human breast cancer using 10366-1-AP

IHC staining of human colon using 10366-1-AP

IHC staining of human hepatocirrhosis using 10366-1-AP

IF Staining of HepG2 using 10366-1-AP

FC experiment of mMSCs using 10366-1-AP

Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

PMID: 31728273

Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.