- Featured Product
- KD/KO Validated
Vimentin Polyclonal antibody
Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
1342
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human liver tissue, human hepatocirrhosis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, human breast cancer tissue, human kidney tissue |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | CoIP, ELISA, FC, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, deer, hamster, pig, bovine |
Immunogen | Vimentin fusion protein Ag0489 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | vimentin |
Synonyms | VIM, vimentin |
Calculated molecular weight | 466 aa, 54 kDa |
Observed molecular weight | 54 kDa |
GenBank accession number | BC000163 |
Gene symbol | VIM |
Uniprot | P08670 |
Gene ID (NCBI) | 7431 |
RRID | AB_2273020 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Vimentin antibody 10366-1-AP | Download protocol |
IHC protocol for Vimentin antibody 10366-1-AP | Download protocol |
IF protocol for Vimentin antibody 10366-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Circ Res Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis. | ||