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- KD/KO Validated
Vimentin Polyclonal antibody
Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
FC, IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
1342
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human liver tissue, human hepatocirrhosis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, human breast cancer tissue, human kidney tissue |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF, IHC, WB, ELISA |
| Cited Applications | CoIP, ELISA, FC, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, deer, hamster, pig, bovine |
| Immunogen | Vimentin fusion protein Ag0489 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vimentin |
| Synonyms | VIM, vimentin |
| Calculated molecular weight | 466 aa, 54 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC000163 |
| Gene symbol | VIM |
| Uniprot | P08670 |
| Gene ID (NCBI) | 7431 |
| RRID | AB_2273020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Vimentin antibody 10366-1-AP | Download protocol |
| IHC protocol for Vimentin antibody 10366-1-AP | Download protocol |
| IF protocol for Vimentin antibody 10366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Circ Res Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis. | ||
