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Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

FC, IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10366-1-AP

Print datasheet

Synonyms

VIM, vimentin



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue, human breast cancer tissue, human liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human breast cancer tissue, human kidney tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, deer, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
UniprotP08670
Gene ID (NCBI) 7431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

实验方案

Product Specific Protocols
WB protocol for Vimentin antibody 10366-1-APDownload protocol
IHC protocol for Vimentin antibody 10366-1-APDownload protocol
IF protocol for Vimentin antibody 10366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Med Sci Monit

PMID: 30605443

Authors - Lin Chen
humanWB

Cytotechnology

Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression.

Authors - Haijun Fan
humanWB

Cancer Sci

Nuclear factor I/B promotes colorectal cancer cell proliferation, epithelial-mesenchymal transition and 5-fluorouracil resistance.

Authors - ZhengYi Liu
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
deerWB

J Proteomics

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Authors - Zhen Dong
mouseWB,IHC

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie