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Vimentin Polyclonal antibody
Vimentin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat No : 10366-1-AP
|Positive WB detected in||HeLa cells, NIH/3T3 cells, Jurkat cells, mouse heart tissue|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue, human breast cancer tissue, human liver tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, human breast cancer tissue, human kidney tissue|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10366-1-AP targets Vimentin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||FC, IF, IHC, WB, ELISA|
|Cited Applications||CoIP, ELISA, FC, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, deer, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Vimentin fusion protein Ag0489|
|Calculated molecular weight||466 aa, 54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC000163|
|Gene ID (NCBI)||7431|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.
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Mesenchymal transition and dissemination of cancer cells is driven by myeloid-derived suppressor cells infiltrating the primary tumor.