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验证数据展示

  • WB analysis of mouse small intestine using 14878-1-AP

    WB analysis of mouse small intestine using 14878-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14878-1-AP (VIPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14878-1-AP

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14878-1-AP

    IHC staining of human kidney using 14878-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14878-1-AP (VIPR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VIPR1 Polyclonal antibody

VIPR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14878-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HVR1, II, PACAP R 2, PACAP R2, PACAP type II receptor, RDC1, VAPC1, VIP R 1, VIPR, VIPR1, VIRG, VPAC1, VPCAP1R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse small intestine tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14878-1-AP targets VIPR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 VIPR1 fusion protein Ag6650 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vasoactive intestinal peptide receptor 1
别名 HVR1, II, PACAP R 2, PACAP R2, PACAP type II receptor, RDC1, VAPC1, VIP R 1, VIPR, VIPR1, VIRG, VPAC1, VPCAP1R
计算分子量 47 kDa
观测分子量50 kDa
GenBank蛋白编号BC064424
基因名称 VIPR1
Gene ID (NCBI) 7433
RRIDAB_2878089
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VIPR1 antibody 14878-1-APDownload protocol
IHC protocol for VIPR1 antibody 14878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

The p65-LOC727924-miR-26a/KPNA3-p65 regulatory loop mediates vasoactive intestinal peptide effects on osteoarthritis chondrocytes

Authors - Yusheng Li
View Article