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VISTA Recombinant antibody

VISTA Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241003A4

Cat no : 83919-4-RR

Print datasheet

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

验证数据展示

  • WB analysis of human peripheral blood platelets using 83919-4-RR

    WB analysis of human peripheral blood platelets using 83919-4-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83919-4-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 83919-4-RR

    IHC staining of human placenta using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VISTA antibody included
  • IHC staining of human placenta using 83919-4-RR

    IHC staining of human placenta using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VISTA antibody included
  • IF Staining of human tonsillitis using 83919-4-RR

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 83919-4-RR

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 83919-4-RR

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83919-4-RR

    Affinity and Kinetic Characterization of 83919-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83919-4-RR against Human VISTA were performed. The affinity constant is 57.9 nM.

经过测试的应用

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83919-4-RR targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 10 open reading frame 54
Synonyms C10orf54, SISP1, PP2135, GI24, B7-H5
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H7M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
WB protocol for VISTA antibody 83919-4-RRDownload protocol
IHC protocol for VISTA antibody 83919-4-RRDownload protocol
IF protocol for VISTA antibody 83919-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols