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验证数据展示

  • IP experiment of SH-SY5Y using 31628-1-AP

    IP experiment of SH-SY5Y using 31628-1-AP

    IP result of anti-VLGR1 (IP:31628-1-AP, 4ug; Detection:31628-1-AP 1:500) with SH-SY5Y cells lysate 1920 ug.

  • WB analysis using 31628-1-AP

    WB analysis using 31628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 31628-1-AP

    WB analysis using 31628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 31628-1-AP

    IF Staining of K-562 using 31628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using VLGR1 antibody (31628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

VLGR1 Polyclonal antibody

VLGR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 31628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

GPR98, USH2B, MASS1, KIAA0686, G protein coupled receptor 98

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31628-1-AP targets VLGR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 VLGR1 fusion protein Ag34635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 98
别名 GPR98, USH2B, MASS1, KIAA0686, G protein coupled receptor 98
计算分子量693 kDa
观测分子量217 kDa
GenBank蛋白编号NM_032119
基因名称 GPR98
Gene ID (NCBI) 84059
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8WXG9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .

实验方案

Product Specific Protocols
WB protocol for VLGR1 antibody 31628-1-APDownload protocol
IF protocol for VLGR1 antibody 31628-1-APDownload protocol
IP protocol for VLGR1 antibody 31628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols