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验证数据展示

  • WB analysis using 66392-1-Ig (same clone as 66392-1-PBS)

    WB analysis using 66392-1-Ig (same clone as 66392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66392-1-Ig (VPRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66392-1-Ig (same clone as 66392-1-PBS)

    WB analysis of HEK-293 using 66392-1-Ig (same clone as 66392-1-PBS)

    WB result of VPRBP antibody (66392-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells. This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
  • IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
  • IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of HepG2 using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 66392-1-Ig (same clone as 66392-1-PBS)

    FC experiment of K-562 using 66392-1-Ig (same clone as 66392-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human VPRBP (66392-1-Ig, Clone:1A7A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

VPRBP Monoclonal antibody, PBS Only

VPRBP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66392-1-PBS

产品说明书

CloneNo. 1A7A8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

产品信息

66392-1-PBS targets VPRBP in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 VPRBP fusion protein Ag2184 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Vpr (HIV-1) binding protein
别名 HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1
计算分子量 1506 aa, 169 kDa
观测分子量 169 kDa
GenBank蛋白编号BC022792
基因名称 VPRBP
Gene ID (NCBI) 9730
RRIDAB_2881768
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4B6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).