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  • Featured Product
  • KD/KO Validated

VPRBP Monoclonal antibody, PBS Only

VPRBP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7A8

Cat no : 66392-1-PBS

Print datasheet

Synonyms

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

验证数据展示

  • WB analysis using 66392-1-Ig (same clone as 66392-1-PBS)

    WB analysis using 66392-1-Ig (same clone as 66392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66392-1-Ig (VPRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66392-1-Ig (same clone as 66392-1-PBS)

    WB analysis of HEK-293 using 66392-1-Ig (same clone as 66392-1-PBS)

    WB result of VPRBP antibody (66392-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells. This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
  • IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IHC staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCAF1/VPRBP antibody included
  • IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of HepG2 using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    IF Staining of human breast cancer using 66392-1-Ig (same clone as 66392-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 66392-1-Ig (same clone as 66392-1-PBS)

    FC experiment of K-562 using 66392-1-Ig (same clone as 66392-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human VPRBP (66392-1-Ig, Clone:1A7A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66392-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

产品信息

66392-1-PBS targets VPRBP in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen VPRBP fusion protein Ag2184 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Vpr (HIV-1) binding protein
Synonyms HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1
Calculated Molecular Weight 1506 aa, 169 kDa
Observed Molecular Weight 169 kDa
GenBank Accession NumberBC022792
Gene Symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4B6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).