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验证数据展示

  • WB analysis using 83901-3-RR (same clone as 83901-3-PBS)

    WB analysis using 83901-3-RR (same clone as 83901-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83901-3-RR (VPRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    IF Staining of HeLa using 83901-3-RR (same clone as 83901-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-3-RR, Clone: 241028B6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83901-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00852-2

    Cytometric bead array standard curve of MP00852-2

    Cytometric bead array standard curve of MP00852-2, VPRBP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83901-3-PBS. Detection antibody: 83901-4-PBS. Standard: Ag2184. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83901-3-RR

    Affinity and Kinetic Characterization of 83901-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83901-3-RR against Human VPRBP were performed. The affinity constant is 0.538 nM.

VPRBP Recombinant antibody, PBS Only (Capture)

VPRBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83901-3-PBS

产品说明书

CloneNo. 241028B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DCAF1/VPRBP Ready-To-Use IHC Kit. DCAF1/VPRBP primary antibody included.

产品信息

83901-3-PBS targets VPRBP as part of a matched antibody pair:

MP00852-2: 83901-3-PBS capture and 83901-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 VPRBP fusion protein Ag2184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Vpr (HIV-1) binding protein
别名 HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1, DCAF1
计算分子量 1506 aa, 169 kDa
观测分子量169 kDa
GenBank蛋白编号BC022792
基因名称 VPRBP
Gene ID (NCBI) 9730
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4B6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).