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Species Reactivity
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验证数据展示

  • WB analysis using 11915-1-AP

    WB analysis using 11915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11915-1-AP (VPREB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

VPREB3 Polyclonal antibody

VPREB3 Polyclonal Antibody for WB, ELISA
Cat No. 11915-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

8HS20, N27C7 2, pre B lymphocyte 3, Pre B lymphocyte protein 3, Protein VPreB3, VPREB3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRamos cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11915-1-AP targets VPREB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen VPREB3 fusion protein Ag2528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pre-B lymphocyte 3
Synonyms 8HS20, N27C7 2, pre B lymphocyte 3, Pre B lymphocyte protein 3, Protein VPreB3, VPREB3
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC020666
Gene Symbol VPREB3
Gene ID (NCBI) 29802
RRIDAB_2216942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPREB3 (formerly named 8HS20) is a protein that associates with mu chains in the endoplasmic reticulum of pre-B cell lines (PMID: 8491176; 22684248). VPREB3 is normally expressed by precursor B cells in bone marrow and by a subset of normal germinal center B cells in secondary lymphoid organs. It has been reported that VPREB3 expression is associated with B-cell tumors with c-MYC abnormalities (PMID: 20823132).

实验方案

Product Specific Protocols
WB protocol for VPREB3 antibody 11915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols