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VPS13A Polyclonal antibody

VPS13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28618-1-AP

Print datasheet

Synonyms

CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A

验证数据展示

  • WB analysis using 28618-1-AP

    WB analysis using 28618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28618-1-AP

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28618-1-AP

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivitymouse
Immunogen VPS13A fusion protein Ag29532 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 13 homolog A (S. cerevisiae)
Synonyms CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A
Calculated Molecular Weight 360 kDa
Observed Molecular Weight360 kDa
GenBank Accession NumberNM_033305
Gene Symbol VPS13A
Gene ID (NCBI) 23230
RRIDAB_2918183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

实验方案

Product Specific Protocols
WB protocol for VPS13A antibody 28618-1-APDownload protocol
IHC protocol for VPS13A antibody 28618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice

Authors - Jitrapa Pinyomahakul
View Article