验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15472-1-AP targets VPS24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | VPS24 fusion protein Ag7776 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 24 homolog (S. cerevisiae) |
| 别名 | hVps24, Chromatin-modifying protein 3, Chromatin modifying protein 3, CHMP3, CGI-149 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC004419 |
| 基因名称 | VPS24 |
| Gene ID (NCBI) | 51652 |
| RRID | AB_2217102 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3E7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IHC protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IF protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IP protocol for VPS24 antibody 15472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
J Cell Biol ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.
| ||
Int J Oncol CHMP3 promotes the progression of hepatocellular carcinoma by inhibiting caspase‑1‑dependent pyroptosis
| ||
J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer |
