验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, human brain tissue, mouse liver tissue, A549 cells, rat liver tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15669-1-AP targets VPS25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, canine |
| 免疫原 | VPS25 fusion protein Ag8134 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 25 homolog (S. cerevisiae) |
| 别名 | hVps25, ESCRT-II complex subunit VPS25, ELL-associated protein of 20 kDa, EAP20, DERP9 |
| 计算分子量 | 176 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC006282 |
| 基因名称 | VPS25 |
| Gene ID (NCBI) | 84313 |
| RRID | AB_2215019 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS25 antibody 15669-1-AP | Download protocol |
| IHC protocol for VPS25 antibody 15669-1-AP | Download protocol |
| IF protocol for VPS25 antibody 15669-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
Nat Commun Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair. | ||
Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. | ||
PLoS Pathog Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection. |
