验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, Jurkat cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15478-1-AP targets VPS28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | VPS28 fusion protein Ag7784 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vacuolar protein sorting 28 homolog (S. cerevisiae) |
| Synonyms | Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC006485 |
| Gene Symbol | VPS28 |
| Gene ID (NCBI) | 51160 |
| RRID | AB_2878144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IF protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IP protocol for VPS28 antibody 15478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody. | ||
iScience VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.
| ||
Curr Protoc Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids | ||
J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer | ||
Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy
|
