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VPS33B Polyclonal antibody

VPS33B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12195-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (4)

Vacuolar protein sorting-associated protein 33B, hVPS33B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, HeLa cells, mouse testis tissue, L02 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12195-1-AP targets VPS33B in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 VPS33B fusion protein Ag2833 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 33 homolog B (yeast)
别名 Vacuolar protein sorting-associated protein 33B, hVPS33B
计算分子量 617 aa, 71 kDa
观测分子量 65-71 kDa
GenBank蛋白编号BC016445
基因名称 VPS33B
Gene ID (NCBI) 26276
RRIDAB_2215198
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H267
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

实验方案

Product Specific Protocols
WB protocol for VPS33B antibody 12195-1-APDownload protocol
IHC protocol for VPS33B antibody 12195-1-APDownload protocol
IF protocol for VPS33B antibody 12195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,IP

Nat Genet

Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.

Authors - Cullinane Andrew R AR
  • KD Validated
mouseWB

Nat Cell Biol

Circadian control of the secretory pathway maintains collagen homeostasis.

Authors - Joan Chang
humanWB,IF

Proc Natl Acad Sci U S A

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Authors - Wong Dennis D
humanIF

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R
humanWB

Clin Kidney J

Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.

Authors - Amelia Holme
humanIHC, WB, CoIP, ChIP

Cancer Sci

VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.

Authors - Yingxia Ning
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