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VPS36 Polyclonal antibody

VPS36 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14262-1-AP

Print datasheet

Synonyms

ELL-associated protein of 45 kDa, EAP45, CGI-145, CGI145, CGI 145

验证数据展示

  • WB analysis of HL-60 using 14262-1-AP

    WB analysis of HL-60 using 14262-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14262-1-AP (VPS36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14262-1-AP

    IHC staining of human pancreas cancer using 14262-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14262-1-AP (VPS36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS36 antibody included

经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman pancreas cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VPS36 Ready-To-Use IHC Kit. VPS36 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14262-1-AP targets VPS36 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen VPS36 fusion protein Ag5549 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 36 homolog (S. cerevisiae)
Synonyms ELL-associated protein of 45 kDa, EAP45, CGI-145, CGI145, CGI 145
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 43 kDa, 65 kDa
GenBank Accession NumberBC050439
Gene Symbol VPS36
Gene ID (NCBI) 51028
RRIDAB_2878036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VPS36 antibody 14262-1-APDownload protocol
IHC protocol for VPS36 antibody 14262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Neurosci Res

Top2b is involved in the formation of outer segment and synapse during late-stage photoreceptor differentiation by controlling key genes of photoreceptor transcriptional regulatory network.

Authors - Ying Li
View Article
humanWB

Cells

Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy.

Authors - Hui-Yung Song
View Article