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VPS37C Polyclonal antibody

VPS37C Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27128-1-AP

Print datasheet

Synonyms

Vacuolar protein sorting-associated protein 37C, PML39, hVps37C, ESCRT-I complex subunit VPS37C, ESCRT I complex subunit VPS37C

验证数据展示

  • WB analysis using 27128-1-AP

    WB analysis using 27128-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27128-1-AP (VPS37C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 27128-1-AP

    IF Staining of Raji using 27128-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using VPS37C antibody (27128-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHT-29 cells, SK-BR-3 cells, T-47D cells
Positive IF/ICC detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27128-1-AP targets VPS37C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen VPS37C fusion protein Ag26024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 37 homolog C (S. cerevisiae)
Synonyms Vacuolar protein sorting-associated protein 37C, PML39, hVps37C, ESCRT-I complex subunit VPS37C, ESCRT I complex subunit VPS37C
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC141827
Gene Symbol VPS37C
Gene ID (NCBI) 55048
RRIDAB_3085928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA5D8V6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS37C (Vacuolar protein sorting-associated protein 37C) is also named as hVps37C, PML39 and ESCRT-I complex subunit VPS37C. VPS37C is a core subunit of the Endosomal sorting complex required for transport-I (ESCRT-I) complex (PMID: 37307706). VPS37C interacts with EKLF and prevents K48-linked polyubiquitination of EKLF and proteasome-mediated EKLF degradation, thus enhancing EKLF protein stability and transcriptional activity (PMID: 37307706).

实验方案

Product Specific Protocols
WB protocol for VPS37C antibody 27128-1-APDownload protocol
IF protocol for VPS37C antibody 27128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols