验证数据展示
经过测试的应用
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human small intestine tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16219-1-AP targets VPS39 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, IHC, ELISA Application Description |
| 文献引用应用 | IP, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | VPS39 fusion protein Ag9267 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 39 homolog (S. cerevisiae) |
| 别名 | hVam6p, KIAA0770, TLP, VAM6, Vam6/Vps39 like protein, VPS39 |
| 计算分子量 | 886 aa, 102 kDa |
| 观测分子量 | 102 kDa |
| GenBank蛋白编号 | BC015817 |
| 基因名称 | VPS39 |
| Gene ID (NCBI) | 23339 |
| RRID | AB_11043179 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96JC1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS39 antibody 16219-1-AP | Download protocol |
| IP protocol for VPS39 antibody 16219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy | ||
Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Dev Cell ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress
| ||
Curr Biol Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. | ||
Cell Rep Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes |
