验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, HepG2 cells, human brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| CoIP | See 1 publications below |
产品信息
13869-1-AP targets VPS41 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | VPS41 fusion protein Ag4760 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 41 homolog (S. cerevisiae) |
| 别名 | HVPS41, hVps41p, HVSP41, S53, VPS41 |
| 计算分子量 | 854 aa, 98 kDa |
| 观测分子量 | 94-100 kDa |
| GenBank蛋白编号 | BC044851 |
| 基因名称 | VPS41 |
| Gene ID (NCBI) | 27072 |
| RRID | AB_2215349 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49754 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. VPS41 (vacuolar protein sorting 41) is the human ortholog of yeast Vps41 protein. Yeast Vps41 is a subunit of AP3 (heterotetrameric adaptor protein complex 3) and HOPS (homotypic fusion and protein transport) complexes and is involved in trafficking of proteins from the late Golgi to the vacuole. In mammalian cells, Vps41 was found to be a member of HOPS complex, mediating the trafficking of proteins to lysosomes (PMID: 23173123). It has been reported that human VPS41 has a HOPS-independent function in the LAMP carrier pathway and also participates in lysosomal maturation, probably as part of the HOPS complex (PMID: 23322049).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS41 antibody 13869-1-AP | Download protocol |
| IHC protocol for VPS41 antibody 13869-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy | ||
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Dev Cell ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation.
| ||
J Cell Sci Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L. | ||
J Biol Chem Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity. | ||
J Cell Sci The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.
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