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验证数据展示

  • IHC staining of mouse brain using 12006-1-AP

    IHC staining of mouse brain using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12006-1-AP

    IHC staining of mouse brain using 12006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12006-1-AP

    IF Staining of HeLa using 12006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12006-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Vacuolar protein sorting-associated protein 45, h-VPS45, hlVps45, H1VPS45, H1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12006-1-AP targets VPS45 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen VPS45 fusion protein Ag2658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Synonyms Vacuolar protein sorting-associated protein 45, h-VPS45, hlVps45, H1VPS45, H1
Calculated Molecular Weight 570 aa, 65 kDa
GenBank Accession NumberBC012932
Gene Symbol VPS45
Gene ID (NCBI) 11311
RRIDAB_2215369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

实验方案

Product Specific Protocols
IHC protocol for VPS45 antibody 12006-1-APDownload protocol
IF protocol for VPS45 antibody 12006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Gene

Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.

Authors - Hui Li
View Article