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VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12616-1-AP

Print datasheet

Synonyms

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A

验证数据展示

  • WB analysis of mouse liver using 12616-1-AP

    WB analysis of mouse liver using 12616-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 12616-1-AP

    IP experiment of mouse kidney using 12616-1-AP

    IP result of anti-VPS45 (IP:12616-1-AP, 4ug; Detection:12616-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

经过测试的应用

Positive WB detected inmouse liver tissue, rat pancreas tissue, human kidney tissue, mouse brain tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12616-1-AP targets VPS45 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VPS45 fusion protein Ag3289 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Synonyms h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
Calculated Molecular Weight 570 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC028382
Gene Symbol VPS45
Gene ID (NCBI) 11311
RRIDAB_2215367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

实验方案

Product Specific Protocols
WB protocol for VPS45 antibody 12616-1-APDownload protocol
IP protocol for VPS45 antibody 12616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.

Authors - Laufman Orly O
View Article