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VPS4A Polyclonal antibody

VPS4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14272-1-AP

Print datasheet

Synonyms

hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A

验证数据展示

  • WB analysis using 14272-1-AP

    WB analysis using 14272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 14272-1-AP

    WB analysis of U-937 using 14272-1-AP

    WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells.

  • IHC staining of human kidney using 14272-1-AP

    IHC staining of human kidney using 14272-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU-937 cells, SMMC-7721 cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14272-1-AP targets VPS4A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Immunogen VPS4A fusion protein Ag5592 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Synonyms hVPS4, Protein SKD2, SKD1, SKD2, VPS4, VPS4 1, VPS4A
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC047932
Gene Symbol VPS4A
Gene ID (NCBI) 27183
RRIDAB_2878039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VPS4A antibody 14272-1-APDownload protocol
IHC protocol for VPS4A antibody 14272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB

Parasitol Res

Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum.

Authors - Francesca De Falco
View Article
humanWB

Theranostics

Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy

Authors - Kunjian Lei
View Article