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VPS4A/B Polyclonal antibody
VPS4A/B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse skin tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17673-1-AP targets VPS4A/B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | VPS4A/B fusion protein Ag11610 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | vacuolar protein sorting 4 homolog B (S. cerevisiae) |
Synonyms | VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1 |
Calculated Molecular Weight | 444 aa, 49 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC039574 |
Gene Symbol | VPS4B |
Gene ID (NCBI) | 9525 |
RRID | AB_2215504 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75351 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for VPS4A/B antibody 17673-1-AP | Download protocol |
IF protocol for VPS4A/B antibody 17673-1-AP | Download protocol |
IP protocol for VPS4A/B antibody 17673-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
EMBO J TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons. | ||
J Cell Sci His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation | ||
Viruses Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding. | ||
J Extracell Vesicles Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles. |