验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, COLO 320 cells, mouse skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11662-2-AP targets VPS52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | VPS52 fusion protein Ag2180 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 52 homolog (S. cerevisiae) |
| 别名 | Vacuolar protein sorting-associated protein 52 homolog, SACM2L, SAC2 suppressor of actin mutations 2-like protein |
| 计算分子量 | 723 aa, 82 kDa |
| 观测分子量 | 82 kDa |
| GenBank蛋白编号 | BC032108 |
| 基因名称 | VPS52 |
| Gene ID (NCBI) | 6293 |
| RRID | AB_2241614 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N1B4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS52 antibody 11662-2-AP | Download protocol |
| IHC protocol for VPS52 antibody 11662-2-AP | Download protocol |
| IF protocol for VPS52 antibody 11662-2-AP | Download protocol |
| IP protocol for VPS52 antibody 11662-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes. | ||
