• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse liver using 12824-1-AP

    WB analysis of mouse liver using 12824-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 12824-1-AP

    IP experiment of mouse liver using 12824-1-AP

    IP result of anti-VPS53 (IP:12824-1-AP, 4ug; Detection:12824-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • IHC staining of human stomach using 12824-1-AP

    IHC staining of human stomach using 12824-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12824-1-AP (VPS53 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VPS53 antibody included

VPS53 Polyclonal antibody

VPS53 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12824-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Vacuolar protein sorting-associated protein 53 homolog, PP13624

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VPS53 Ready-To-Use IHC Kit. VPS53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12824-1-AP targets VPS53 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 VPS53 fusion protein Ag3518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 53 homolog (S. cerevisiae)
别名 Vacuolar protein sorting-associated protein 53 homolog, PP13624
计算分子量 832 aa, 94 kDa
观测分子量 95-97 kDa
GenBank蛋白编号BC029560
基因名称 VPS53
Gene ID (NCBI) 55275
RRIDAB_2215514
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VIR6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

实验方案

Product Specific Protocols
WB protocol for VPS53 antibody 12824-1-APDownload protocol
IHC protocol for VPS53 antibody 12824-1-APDownload protocol
IP protocol for VPS53 antibody 12824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

Biallelic variants in VPS50 cause a neurodevelopmental disorder with neonatal cholestasis.

Authors - Pauline E Schneeberger
View Article