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VPS53 Polyclonal antibody

VPS53 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12824-1-AP

Print datasheet

Synonyms

Vacuolar protein sorting-associated protein 53 homolog, PP13624

验证数据展示

  • WB analysis of mouse liver using 12824-1-AP

    WB analysis of mouse liver using 12824-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12824-1-AP (VPS53 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 12824-1-AP

    IP experiment of mouse liver using 12824-1-AP

    IP result of anti-VPS53 (IP:12824-1-AP, 4ug; Detection:12824-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • IHC staining of human stomach using 12824-1-AP

    IHC staining of human stomach using 12824-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12824-1-AP (VPS53 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, L02 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12824-1-AP targets VPS53 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VPS53 fusion protein Ag3518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Synonyms Vacuolar protein sorting-associated protein 53 homolog, PP13624
Calculated Molecular Weight 832 aa, 94 kDa
Observed Molecular Weight 95-97 kDa
GenBank Accession NumberBC029560
Gene Symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2215514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VIR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

实验方案

Product Specific Protocols
WB protocol for VPS53 antibody 12824-1-APDownload protocol
IHC protocol for VPS53 antibody 12824-1-APDownload protocol
IP protocol for VPS53 antibody 12824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

Biallelic variants in VPS50 cause a neurodevelopmental disorder with neonatal cholestasis.

Authors - Pauline E Schneeberger
View Article