验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, NIH/3T3 cells, A549 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human breast hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
15143-1-AP targets VPS72 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | VPS72 fusion protein Ag7424 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vacuolar protein sorting 72 homolog (S. cerevisiae) |
| Synonyms | CFL1, Protein YL 1, Swc2, TCFL1, Transcription factor like 1, VPS72, YL 1, YL1 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC003151 |
| Gene Symbol | VPS72 |
| Gene ID (NCBI) | 6944 |
| RRID | AB_2215534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15906 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex, which exists in Drosophila melanogaster and mammalian cells, is a complex that is responsible for various cellular processes, including DNA repair, transcriptional activation and apoptosis. Vacuolar protein sorting-associated protein 72 homolog (VPS72), also known as VPS72 or Transcription factor-like 1, is a 364 amino acid subunit of the TRRAP/TIP60 HAT complex. VPS72 has also been identified as a subunit of a novel complex containing SNF2-related helicase SRCAP (SWI2/SNF2-related CBP activator protein). This SRCAP-containing complex is very similar to the S. cerevisiae SWR1 chromatin remodeling complex. The involvement of VPS72 in these complexes has suggested that VPS72 plays multiple roles in chromatin modification and remodeling in cells. VPS72 localizes to the nucleus and is phosphorylated upon DNA damage, most likely by ATM or ATR.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS72 antibody 15143-1-AP | Download protocol |
| IHC protocol for VPS72 antibody 15143-1-AP | Download protocol |
| IP protocol for VPS72 antibody 15143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Gen Med Expression Analysis of VPS72 and Associated Biological Behaviors in Colon Cancer |
