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  • Featured Product
  • KD/KO Validated

VPS8 Polyclonal antibody

VPS8 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15079-1-AP

Print datasheet

Synonyms

Vacuolar protein sorting-associated protein 8 homolog, KIAA0804

验证数据展示

  • WB analysis of human brain using 15079-1-AP

    WB analysis of human brain using 15079-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15079-1-AP (VPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15079-1-AP

    IF Staining of HepG2 using 15079-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15079-1-AP (VPS8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse brain using 15079-1-AP

    IP experiment of mouse brain using 15079-1-AP

    IP result of anti-VPS8 (IP:15079-1-AP, 4ug; Detection:15079-1-AP 1:600) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman brain tissue, human testis tissue, mouse brain tissue, mouse liver tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15079-1-AP targets VPS8 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VPS8 fusion protein Ag7151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vacuolar protein sorting 8 homolog (S. cerevisiae)
Synonyms Vacuolar protein sorting-associated protein 8 homolog, KIAA0804
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 151 kDa
GenBank Accession NumberBC001001
Gene Symbol VPS8
Gene ID (NCBI) 23355
RRIDAB_2215543
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N3P4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS8, also named as KIAA0804, has some isoforms with MW 135-160 kDa and 70-90 kDa.

实验方案

Product Specific Protocols
WB protocol for VPS8 antibody 15079-1-APDownload protocol
IF protocol for VPS8 antibody 15079-1-APDownload protocol
IP protocol for VPS8 antibody 15079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.

Authors - Dawei Wang
  • KD Validated
View Article
humanWB, IP

Traffic

Mammalian CORVET is required for fusion and conversion of distinct early endosome sub-populations.

Authors - Enrico Daniele Perini
View Article
humanWB

Biochem J

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

Authors - Morag R Hunter
View Article
humanWB

Sci Rep

HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis

Authors - Seigo Terawaki
  • KO Validated
View Article