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验证数据展示

  • WB analysis using 85598-2-RR (same clone as 85598-2-PBS)

    WB analysis using 85598-2-RR (same clone as 85598-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85598-2-RR (VRK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85598-2-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 85598-2-RR (same clone as 85598-2-PBS)

    IF Staining of U2OS using 85598-2-RR (same clone as 85598-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VRK1 antibody (85598-2-RR, Clone: 242978A1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85598-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01967-1

    Cytometric bead array standard curve of MP01967-1

    Cytometric bead array standard curve of MP01967-1, VRK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85598-3-PBS. Detection antibody: 85598-2-PBS. Standard: Ag27754. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01967-2

    Cytometric bead array standard curve of MP01967-2

    Cytometric bead array standard curve of MP01967-2, VRK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85598-1-PBS. Detection antibody: 85598-2-PBS. Standard: Ag27754. Range: 0.313-40 ng/mL.

VRK1 Recombinant antibody, PBS Only (Detector)

VRK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85598-2-PBS

产品说明书

CloneNo. 242978A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85598-2-PBS targets VRK1 as part of a matched antibody pair:

MP01967-1: 85598-3-PBS capture and 85598-2-PBS detection (validated in Cytometric bead array)

MP01967-2: 85598-1-PBS capture and 85598-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 VRK1 fusion protein Ag27754 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vaccinia related kinase 1
别名
计算分子量 396 aa, 45 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC103761
基因名称 VRK1
Gene ID (NCBI) 7443
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99986
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vaccinia-related kinase 1 (VRK1) is a members of the vaccinia-related kinase (VRK) family of serine/threonine kinases and are principally localized in the nucleus. VRK1 was initially described as a kinase with homology to B1R, a vaccinia virus kinase required for viral DNA replication and the early phase of infection. It has been suggested that VRK1 was overexpressed in numerous human tumors, such as lung carcinomas, hepatocellular carcinoma, breast carcinomas, head and neck squamous cell carcinoma. (PMID: 28927264, PMID: 29103766)