VILIP-1 Polyclonal antibody

VILIP-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13919-1-AP

Print datasheet

Synonyms

VILIP 1, VILIP1, VSNL1, VILIP, HUVISL1



经过测试的应用

Positive WB detected inmouse brain tissue, mouse tongue tissue, human brain tissue, rat brain tissue, HeLa cells, mouse cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inHuman bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13919-1-AP targets VILIP-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen VILIP-1 fusion protein Ag4923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name visinin-like 1
Synonyms VILIP 1, VILIP1, VSNL1, VILIP, HUVISL1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-24 kDa
GenBank Accession NumberBC022012
Gene Symbol VSNL1
Gene ID (NCBI) 7447
RRIDAB_2215851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VILIP-1 antibody 13919-1-APDownload protocol
IHC protocol for VILIP-1 antibody 13919-1-APDownload protocol
IF protocol for VILIP-1 antibody 13919-1-APDownload protocol
IP protocol for VILIP-1 antibody 13919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouseWB,IF

Free Radic Biol Med

Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.

Authors - Martina P Liebl
mouseWB

Front Cell Neurosci

Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.

Authors - Anna Sancho-Balsells