VSX2 Polyclonal antibody
VSX2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse eye tissue |
Positive IHC detected in | human retinoblastoma tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse eye tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
25825-1-AP targets VSX2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | VSX2 fusion protein Ag23035 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | visual system homeobox 2 |
Synonyms | MCOPCB3, MCOP2, HOX10, Homeobox protein CHX10, CHX10 |
Calculated Molecular Weight | 361 aa, 39 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC128153 |
Gene Symbol | VSX2 |
Gene ID (NCBI) | 338917 |
RRID | AB_2880257 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P58304 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for VSX2 antibody 25825-1-AP | Download protocol |
IHC protocol for VSX2 antibody 25825-1-AP | Download protocol |
IF protocol for VSX2 antibody 25825-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia |