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验证数据展示

  • IF Staining of mouse eye using 25825-1-AP

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse eye using 25825-1-AP

    WB analysis of mouse eye using 25825-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human retinoblastoma using 25825-1-AP

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human retinoblastoma using 25825-1-AP

    IHC staining of human retinoblastoma using 25825-1-AP

    Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 25825-1-AP

    IF Staining of mouse eye using 25825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VSX2 Polyclonal antibody

VSX2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 25825-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, IF-Fro, ELISA and More (1)

别名

Ceh-10 homeodomain-containing homolog, CHX10, Homeobox protein CHX10, HOX10, MCOP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inhuman retinoblastoma tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF-Fro detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

25825-1-AP targets VSX2 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 VSX2 fusion protein Ag23035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 visual system homeobox 2
别名 Ceh-10 homeodomain-containing homolog, CHX10, Homeobox protein CHX10, HOX10, MCOP2
计算分子量 361 aa, 39 kDa
观测分子量 42 kDa
GenBank蛋白编号BC128153
基因名称 VSX2
Gene ID (NCBI) 338917
RRIDAB_2880257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58304
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VSX2 antibody 25825-1-APDownload protocol
IHC protocol for VSX2 antibody 25825-1-APDownload protocol
IF protocol for VSX2 antibody 25825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Human brain organoids assemble functionally integrated bilateral optic vesicles.

Authors - Elke Gabriel
View Article
humanIF

Nat Protoc

Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia

Authors - Elke Gabriel
View Article