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VSX2 Monoclonal antibody

VSX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C7C1

Cat no : 67626-1-Ig

Print datasheet

Synonyms

CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2

验证数据展示

  • WB analysis using 67626-1-Ig

    WB analysis using 67626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67626-1-Ig (VSX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of rat eye using 67626-1-Ig

    WB analysis of rat eye using 67626-1-Ig

    rat eye tissue were subjected to SDS PAGE followed by western blot with 67626-1-Ig (VSX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRaji cells, rat eye tissue, Ramos cells, Daudi cells, NCCIT cells, A549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67626-1-Ig targets VSX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen VSX2 fusion protein Ag29867 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name visual system homeobox 2
Synonyms CHX10, Homeobox protein CHX10, HOX10, MCOP2, MCOPCB3, RET1, visual system homeobox 2, VSX2
Calculated Molecular Weight 361 aa, 39 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC128153
Gene Symbol VSX2
Gene ID (NCBI) 338917
RRIDAB_2882827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP58304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VSX2, also named as CHX10 and HOX10, belongs to the paired homeobox family. VSX2 plays a significant role in the specification and morphogenesis of the sensory retina. It may also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells. Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2). Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI). Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3). The antibody is specific to VSX2.

实验方案

Product Specific Protocols
WB protocol for VSX2 antibody 67626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols