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VTA1 Polyclonal antibody

VTA1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15786-1-AP

Print datasheet

Synonyms

HSPC228, DRG-1, DRG1, DRG 1, Dopamine-responsive gene 1 protein

验证数据展示

  • WB analysis using 15786-1-AP

    WB analysis using 15786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15786-1-AP

    WB analysis using 15786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15786-1-AP

    IF Staining of HeLa using 15786-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IP experiment of HEK-293 using 15786-1-AP

    IP experiment of HEK-293 using 15786-1-AP

    IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:10000) with HEK-293 cells lysate 1440 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HepG2 cells, human urine sample, K-562 cells, rat brain tissue, HSC-T6 cells, SMMC-7721 cells, mouse brain tissue, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15786-1-AP targets VTA1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen VTA1 fusion protein Ag8485 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Vps20-associated 1 homolog (S. cerevisiae)
Synonyms HSPC228, DRG-1, DRG1, DRG 1, Dopamine-responsive gene 1 protein
Calculated Molecular Weight 307 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC006989
Gene Symbol VTA1
Gene ID (NCBI) 51534
RRIDAB_2288524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VTA1 antibody 15786-1-APDownload protocol
IF protocol for VTA1 antibody 15786-1-APDownload protocol
IP protocol for VTA1 antibody 15786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic analyses uncover a new function and mode of action for mDia2.

Authors - Tadamoto Isogai
View Article