验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, pig plasma tissue, rabbit serum tissue |
| Positive IHC detected in | human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy VTN Ready-To-Use IHC Kit. VTN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
66398-1-Ig targets VTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, pig, rabbit, mouse |
| 文献引用反应性 | pig |
| 免疫原 | VTN fusion protein Ag8443 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vitronectin |
| 别名 | S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN |
| 计算分子量 | 478 aa, 54 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC005046 |
| 基因名称 | VTN |
| Gene ID (NCBI) | 7448 |
| RRID | AB_2881772 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P04004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VTN antibody 66398-1-Ig | Download protocol |
| IHC protocol for VTN antibody 66398-1-Ig | Download protocol |
| IF protocol for VTN antibody 66398-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
