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VWA2 Polyclonal antibody

VWA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18714-1-AP

Print datasheet

Synonyms

von Willebrand factor A domain-containing protein 2, NET42, CCSP 2, AMACO, A domain-containing protein similar to matrilin and collagen

验证数据展示

  • WB analysis of A375 using 18714-1-AP

    WB analysis of A375 using 18714-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18714-1-AP (VWA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 18714-1-AP

    IHC staining of human colon using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with VWA2 antibody included
  • IHC staining of human colon using 18714-1-AP

    IHC staining of human colon using 18714-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18714-1-AP (VWA2 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with VWA2 antibody included
  • IF Staining of HEK-293 using 18714-1-AP

    IF Staining of HEK-293 using 18714-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using VWA2 antibody (18714-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inA375 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, A375 cells
Planning an IHC experiment? We recommend our IHCeasy VWA2 Ready-To-Use IHC Kit. VWA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18714-1-AP targets VWA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name von Willebrand factor A domain containing 2
Synonyms von Willebrand factor A domain-containing protein 2, NET42, CCSP 2, AMACO, A domain-containing protein similar to matrilin and collagen
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC128588
Gene Symbol VWA2
Gene ID (NCBI) 340706
RRIDAB_10598772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5GFL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VWA2, also named as AMACO and CCSP2, is a novel candidate serological marker of colon neoplasia. VWA2 is found in the media of variesy of established cell lines of both fibroblast and epithelial origin. This antibody can recognize all the 3 isoforms of VWA2.

实验方案

Product Specific Protocols
WB protocol for VWA2 antibody 18714-1-APDownload protocol
IHC protocol for VWA2 antibody 18714-1-APDownload protocol
IF protocol for VWA2 antibody 18714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols