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Vaspin Monoclonal antibody

Vaspin Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1B6

Cat no : 67573-1-Ig

Print datasheet

Synonyms

SERPINA12, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12, OL-64, OL 64

验证数据展示

  • WB analysis using 67573-1-Ig

    WB analysis using 67573-1-Ig

    Human adipose tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67573-1-Ig

    IF Staining of HeLa using 67573-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vaspin antibody (67573-1-Ig, Clone: 2A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inhuman adipose tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67573-1-Ig targets Vaspin in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Vaspin fusion protein Ag30136 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Synonyms SERPINA12, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12, OL-64, OL 64
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC040857
Gene Symbol Vaspin
Gene ID (NCBI) 145264
RRIDAB_2882786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IW75
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Vaspin antibody 67573-1-IgDownload protocol
IF protocol for Vaspin antibody 67573-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols