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VEGFA Recombinant antibody

VEGFA Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5

Cat no : 82864-16-RR

Print datasheet

Synonyms

Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

验证数据展示

  • WB analysis using 82864-16-RR

    WB analysis using 82864-16-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82864-16-RR (Vegfa antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82864-16-RR

    Affinity and Kinetic Characterization of 82864-16-RR

    Biolayer interferometry (BLl) kinetic assays of 82864-16-RR against Mouse VEGFA were performed. The affinity constant is 0.505 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, MDA-MB-231 cells, HUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82864-16-RR targets VEGFA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor
Observed Molecular Weight38 kDa
GenBank Accession NumberNM_001287056
Gene Symbol Vegfa
Gene ID (NCBI) 22339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 82864-16-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols