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Vegfa Recombinant antibody

Vegfa Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2I18

Cat no : 82864-7-RR

Print datasheet

Synonyms

Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

验证数据展示

  • IF Staining of NIH/3T3 using 82864-7-RR

    IF Staining of NIH/3T3 using 82864-7-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82864-7-RR

    Affinity and Kinetic Characterization of 82864-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:220-1:880
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82864-7-RR targets Vegfa in IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms Vpf, Vegf a, Vegf, vascular endothelial growth factor A, Vascular Endothelial Growth Factor
GenBank Accession NumberNM_001287056
Gene Symbol Vegfa
Gene ID (NCBI) 22339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

实验方案

Product Specific Protocols
IF protocol for Vegfa antibody 82864-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols