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Villin Monoclonal antibody, PBS Only

Villin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7B9

Cat no : 66096-1-PBS

Print datasheet

Synonyms

VIL1, villin 1, VIL, D2S1471, 2B7B9

验证数据展示

  • WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
  • IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66096-1-Ig (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.

产品信息

66096-1-PBS targets Villin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Villin fusion protein Ag9637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name villin 1
Synonyms VIL1, villin 1, VIL, D2S1471, 2B7B9
Calculated Molecular Weight827aa,93 kDa; 826aa,93 kDa
Observed Molecular Weight 93-95 kDa
GenBank Accession NumberBC017303
Gene Symbol Villin
Gene ID (NCBI) 7429
RRIDAB_2881495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.