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Villin Monoclonal antibody, PBS Only

Villin Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B7B9

Cat No : 66096-1-PBS

Print datasheet

Synonyms

VIL, VIL1, Villin, villin 1

验证数据展示

  • WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    WB analysis of human kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66096-1-Ig (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    IF Staining of HepG2 using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66096-1-Ig (Villin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon cancer using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66096-1-Ig (Villin antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
  • IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP experiment of mouse kidney using 66096-1-Ig (same clone as 66096-1-PBS)

    IP result of anti-Villin (IP:66096-1-Ig, 4ug; Detection:66096-1-Ig 1:1000) with mouse kidney tissue lysate 6000ug. This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66096-1-Ig (same clone as 66096-1-PBS)

    IHC staining of human colon cancer using 66096-1-Ig (same clone as 66096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66096-1-Ig (Villin antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VIL1 antibody included
Planning an IHC experiment? We recommend our IHCeasy VIL1 Ready-To-Use IHC Kit. VIL1 primary antibody included.

产品信息

66096-1-PBS targets Villin in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen Villin fusion protein Ag9637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name villin 1
Synonyms VIL, VIL1, Villin, villin 1
Calculated Molecular Weight827aa,93 kDa; 826aa,93 kDa
Observed Molecular Weight 93-95 kDa
GenBank Accession NumberBC017303
Gene Symbol VIL1
Gene ID (NCBI) 7429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09327
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Villin 1 (VIL1) is a 95-kDa F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.