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ChromoTek Vimentin-Label Atto488

anti-vimentin VHH/ Nanobody conjugated to the fluorophore ATTO488 for IF, & microscopy

Specificity

Vimentin

Applications

IF, ICC

Conjugate

ATTO 488

Host

Alpaca

Cat No : vba488

Synonyms

Vimentin-Label, VIM, Vimentin

Validation Data Gallery

  • MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).

    MDCK cells were immunostained with Vimentin-Booster (in green), cell nuclei were counterstained with DAPI (in blue).

Product Information

anti-vimentin VHH/ Nanobody conjugated to the fluorophore ATTO488 for IF, & microscopy

DescriptionVimentin-Label specifically binds to the Vimentin intermediate filament protein. Due to its small size, the Vimentin-Labelenables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Specific probe for direct immunostaining of Vimentin filaments

• Higher image resolution

• Less than 4 nm epitope-label displacement minimizes linkage error

• Superior accessibility and labeling of epitopes in crowded cellular/organelle environments

• Improved target binding due to an avidity effect from the bivalent form of Vimentin-Label

ApplicationsMonoclonal
HostAlpaca
Specificity/TargetVimentin-Label specifically binds to the vimentin intermediate filament protein. Validated on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ.
ConjugateATTO 488
Physical StateLiquid
Suggested DilutionIF/ICC: 1:200 – 1:400
Type Nanobody
ClassRecombinant
RRIDAB_2631392
Storage Buffer1x PBS, 0.09% sodium azide.
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Nat Neurosci

Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull-meninges connections.

Authors - Ruiyao Cai
View Article
IF

Light Sci Appl

Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy.

Authors - Jing Liu
View Article